Select ALL of the reasons that fluorescence spectrometry have particularly low d
ID: 556965 • Letter: S
Question
Select ALL of the reasons that fluorescence spectrometry have particularly low detection limits for analytes that fluoresce.
Select ALL of the reasons that fluorescence spectrometry have particularly low detection limits for analytes that fluoresce. Fluorescence causes a small change in a big signal that is detected at low concentrations Fluorescence calibration curves often have very steep calibration curves. Fluorescent analytes often do not absorb much light in either the UV or the visible range. Fluorescence emission is measured against a dark background. Fluorescence calibration curves always bend towards the concentration axis at high concentrations of analyte.Explanation / Answer
Answer:
1. fluorescence spectrometry causes small change in big signal that is detected at low concentration.
2.Fluorecent analytes often do not absorb much light in either the UV or the visible range.
3.Fluorescence emission is measured against a dark background.
4.fluorescence calibration curves always bend towards the concentration axis at high concentration of analyte.