A culture of E. coli (leu+, thr+, azi-r) is grown and infected by the lysogenic

Question

A culture of E. coli (leu+, thr+, azi-r) is grown and infected by the lysogenic phage P1. The resulting lysate is used to infect a similar culture of E. coli (leu-, thr-, azi-s). Cells that survive the infection are plated on minimal medium and on medium supplemented with either leucine or threonine. In this way, bacteria carrying leu+, thr+, or both can be selected and tested for the presence of other markers. Which two markers are closely linked? What is the linear order of the three markers? The data suggest that the region thr-leu represents the extreme length of donor fragment which can be transduced by P1. Why?

Explanation / Answer

a] leu and azi are closely linked.

b] thre+ leu+ azi+

c] Because they are distantly located.

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