Consider the \"snapshot\" of the chymotrypsin mechanism shown below. What is the

Question

Consider the "snapshot" of the chymotrypsin mechanism shown below. What is the next step that will happen to complete this mechanism? His57 Ser195 Asp102 Rv Oi The negatively charged oxygen will abstract a hydrogen tas a proton) from a water molecule: the resulting OH will i abstract a hydrogen (as a proton) ,,orn His57 A water molecule willattack the metylere of Se 195. This attack will tead to a severing of the band between the Ser A water molecule will donate a proton o the negatively charged oxygen, thus forming OH which will attack the 195 oxygen and the tetrahedral carbon this e 1 evering in tin will allow the tydroxyl on Ser 195 to re form o methylene df Se 195 one of the other choices prewides areaso exbiariation of the next steo of the reaction A water ule will attack thei ben

Explanation / Answer

The COOR-group of Asp102 in chymotrypsin will form a H-bond with histidine. After this the hydrogen bond shifts the electron density to the other nitrogen atom which in turn becomes a very strong base. This will further deprotonate Ser195 and turn it into a strong nucleophile that can attack the substrate.

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