Question 1 & Calculations(6 p). You will need a metric ruler (NOT inches]. First
ID: 579202 • Letter: Q
Question
Question 1 & Calculations(6 p). You will need a metric ruler (NOT inches]. First state the equation that gives the retention factor, R. Next, for spots "x" "Y" and "2" in the chramatogram belaw, calculate theR This will involve taking measurements directly off the chromatogram. Show all work. You may 'ind page 6 af the Paper Chromatogrophy technical guide helpful. Control oiisig figs! origin Question 2 Paper Chromatography Analysis (4 p. Four pure substances leach has a different color: blue, yellow, orange and red) are run with five different mobile phases by paper chromatography: pure solvent A, pure sglvent, and various mixtures of solvent "A" and "B" as indicated in the diagrams below. The paper chromatography was carried out as per the Paper Chromatography technical guide. Carefully study the results. There are three things to consider: 1 the sharper(less spread-out) the spot the better, 2] the·center of mass. height of each spot should be different-the greater the difference the better, and 3), the development time {should be 15 minutes or less). based on 1) through 3) what is the best mobile phase that resolves all four substances? Solvent Front Origin Pure SolventA 5 minutes 3:1 (75/25) A to B 6 minutes 1:1 (50150) A to B 9 minutes 1:3 (25/75) A to B 15 minutes Pure Solvent B 32 minutesDevelopment Time Considering the above results, what solvent "A8 ratio would be appropriate to try next in an effort to find the best mobile phase to resolve all four substances?Explanation / Answer
Pure solvent A: not good choice because all the spots are very much spread out.
3:1 (75/25) A to B: not good as the diffrence between the height of spots are not significant and therse are not sharper too
1:1 (50/50) : good choice because these spots are sharper and there are significant difference in height for spots. Also, the development time is less than 15 minutes
1:3: not good, as the diffrence between the height of spots are not significant
pure solvent: not good, as it is very much slow process
so, the good choice for next try is 1:1 (50/50) A to B