Matching details about various techniques used in isolating proteins with the ap
ID: 580187 • Letter: M
Question
Matching details about various techniques used in isolating proteins with the appropriate technique. Note that more than one technique may match with more than on statement below.
A.Isoelectric Focusing
B Xray crystallography
C. Tryptic mapping
D. Biosensors
E. Spectroscopy
F. Mass spectrometry
G. Analytical Ultracentrifugation
1. A technique by which the mass of a protein can be determined with remarkable precision of the order of a few hydrogen atoms.
2. In this technique small peptides derived from the protein by endoprotease action are separated by high resolution reverse phase HPLC; the individual peptides are then subjected to sequencing to confirm the identity of the given peptide.
3. Device used for the detection of protein cells.
4. This technique is used for characterizing metal containing protein cofactors.
5. A technique by which addition of sample containing a particular protein will produce an immediate signal in the presence of an immobilized antibody to that particular protein
6. In this technique separation is based on charge, and can be used to experimentally determine pI
7. This technique allows measurement of a variety of properties of a protein sample, including solution molecular weight, interaction with other molecules and sample homogeneity.
8. A technique by which any important modification (post translational modification) or variation in a protein structure can be detected.
9. In this technique electrons in crystal scatter x-rays to produce an image which can be converted into 3-D structure.
10. This technique gives an indication of the fraction of the polypeptide that is composed of specific secondary structural features such as -helix and -sheet.
Explanation / Answer
A.Isoelectric Focusing = 6
B Xray crystallography = 9
C. Tryptic mapping = 2
D. Biosensors = 5, 3
E. Spectroscopy = 10
F. Mass spectrometry = 1, 9, 4
G. Analytical Ultracentrifugation = 7