Choose the correct answer for each: 1. The main advantage of fluorescence over a
ID: 587161 • Letter: C
Question
Choose the correct answer for each:
1. The main advantage of fluorescence over absorbance spectroscopy is?
a). Fluorescence is a zero-background technique
b). Fluorescence is more sensitive
2. The main advantage of an array detector over a single channel detector (such as photmultiplier) is?
a). An array detector can analyze many wavelengths simultaneously
b). A single channel detector analyzes many wavelengths simultaneously
3. Fluorescence emission is collected at right angles to the incident beam in order to?
a). Minimize detection of incident radiation
b). Maximize detection of incident radiation
4. The resolving power of a diffraction grating?
a). Decreases with the number of illuminated grooves
b). Increases with the number of illuminated grooves
Explanation / Answer
Ans 1 : B) Fluorescence is more sensitive
Fluorescence sensitivity is approximately 1000 times more than the absorbance spectrometry. This causes the use of less sample along with the greater limits of detection.