For the partial diploid you\'ve created, F\' I^+ P^+ O^c Z^+ Y^-/I^- P^+ O^+ Z^-
ID: 59266 • Letter: F
Question
For the partial diploid you've created, F' I^+ P^+ O^c Z^+ Y^-/I^- P^+ O^+ Z^- Y^-, you already know that the promoter sequences for copies are functional, so you can focus on the repressor proteins and operator regions that control expression. Indicate the source of repressor protein and the operator region(s) to which it binds. Then indicate how this affects expression of lac genes from the F' plasmid and from the bacterial chromosome. the F' plasmid the bacterial chromosome both the F' plasmid and the chromosome neither the F' plasmid nor the chromosome Repressor protein is made from. Repressor protein is able to bind to the operator region on. Functional beta-galactosidase protein could be made from. Functional permease protein could be made from.Explanation / Answer
* Repressor is made from F' plasmid only. The gene for repressor on the chromosome is non-functional
* Repressor protein is both Cis and Trans acting. However, the operator on F' plasmid is a constitutive mutant and cannot bind the repressor. Only the operator region of the chromosome binds to the repressor. Therefore, correct answer is the bacterial chromosome
* Functional beta-galactosidase could be made from F' plasmid. The chromosome has a mutant gene for beta-galactosidase.
* Permease gene is not functional in the F' plasmid (mutated). Though there is a functional gene in the chormosome, the promotor is bound by the repressor, and hence is not transcribed. Therefore, the permease is not produced from either of the genes. The correct answer is neither the F' plasmid nor the chromosome.