The table at the right gives the molecular weight, diffusion coefficient, and pl
ID: 596419 • Letter: T
Question
The table at the right gives the molecular weight, diffusion coefficient, and pl for three proteins. You are trying to decide the best methods for separating the proteins from each other. Identify the protein which matches each behavior or property listed below. Elutes first from a gel filtration column. Elutes first from a diethylaminoethyl cellulose ion exchange column with a decreasing pH gradient. Elutes first from a carboxymethyl cellulose ion exchange column with an increasing pH gradient. Migrates fastest on SDS gel electrophoresis. Has the largest frictional coefficient. Migrates to the anode upon electrophoresis at pH 4.9. Migrates to the cathode upon electrophoresis at pH 7.0. Note: If you answer this question incorrectly, a single red X will appear indicating that one or more of the blanks are incorrect.Explanation / Answer
E