An octapeptide with the amino acids was subjected to digestion reactions A, B an

Question

An octapeptide with the amino acids was subjected to digestion reactions A, B and C in order to deduce its amino acid sequence. The following results were obtained. The exact position occupied by each of the amino acids (positions 1-8 in the sequence) will be known when the results are analyzed A. Digestion with Trypsin yielded a [Thr-Arg] dipeptide and a [hexapeptide] with the remaining amino acids.

Question (A1): Explain the reasoning for the fragmentation pattern

Question (A2): What can you deduce regarding the exact positions occupied by Thr and Arg in the

sequence?

Explanation / Answer

A1. Trypsin cleaves the polypeptide at the carboxy terminus of Lys or Arg except when either is bound to C termini proline.

A2. From this pattern, we can infer that Thr and Arg are present at the C-termini of the polypeptide and there is no Proline residue after the Arg residue.

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