In endpoint PCR the following steps describe the correct order of a PCR program:
ID: 61259 • Letter: I
Question
In endpoint PCR the following steps describe the correct order of a PCR program:
Denaturing of duplex DNA, dissociation of polymerase, annealing of primers, repeat
Denaturing of duplex DNA, annealing of primers, elongation by a polymerase, repeat
Denature of polymerase, annealing of duplex DNA, dissociation of elongase, repeat
Dissociation of reverse transcriptase, elongation by a primer, annealing of polymerase,
repeat
Denaturing of duplex DNA, dissociation of polymerase, annealing of primers, repeat
Denaturing of duplex DNA, annealing of primers, elongation by a polymerase, repeat
Denature of polymerase, annealing of duplex DNA, dissociation of elongase, repeat
Dissociation of reverse transcriptase, elongation by a primer, annealing of polymerase,
repeat
Explanation / Answer
The endpoint PCR measures the total amount of amplified DNA at the PCR reaction. It involves following steps per one cycle:
The correct order of endpoint PCR program is as follows:
Denaturing of duplex DNA, annealing of primers, elongation by a polymerase, repeat
Thus, the correct option is (b).