Can someone please help me answer the questions above and explain why did you pi
ID: 61616 • Letter: C
Question
Can someone please help me answer the questions above and explain why did you pick that answer?
Thiolase catalyzes the following reaction: Acetylacetyl-Co + Coenzyme A rightarow 2 Acetyl CoA Below are plots for possible result based on the inhibitor HSCoA. Answer the following 3 questions based on the above plots: Shows that the HSCoA inhibitor is competitive. Shows that the HSCoA inhibitor shows mixed inhibition. For the above plots, if instead of the inhibitor, the increasing species was either AcetylAcetyl-Co or Coenzyme A, which indicates that the reaction follows a sequential mechanism For the above plots, if instead of the inhibitor, the increasing species was either AcetylAcetyl-Co or Coenzyme A, which indicates that the reaction follows a ping pong mechanismExplanation / Answer
In competitive inhibition, Vmax remains constant and Km increases. None of the above plots is showing constant Vmax. So, inhibition is not competitive.
HSCoA inhibitor is mixed inhibition in which Vmax decreases.
Plot B is showing ping-pong mechanism (please note: it is non sequential). When Aceto acetyl CoA binds to enzyme product is formed. This product binds to second reactant to form the final product.
Plot D is showing sequential mechanism