Problem 16.22 A marine bacterium is isolated and shown to contain an inducible o

Question

Problem 16.22 A marine bacterium is isolated and shown to contain an inducible operon whose genetic products metabolize oil when it is encountered in the environment. Investigation demonstrates that the operon is under positive control and that there is a reg gene whose product interacts with an operator region (o) to regulate the structural genes, designated sg. ? In an attempt to understand how the operon functions, a constitutive mutant strain and several partial diploid strains were isolated and tested with the results shown here.

Part A

In this system, the presence of oil

Part B

The regulatory molecule is

Part C

Is the constitutive mutation in the trans-acting reg element or in the cis-acting ooperator element?

Explanation / Answer

Part A

In this system, the presence of oil induces the expression of the operon.

Part B

In this system, the regulatory molecule is an activator, it stimulates transcription.

Part C

The operon with the mutant strain shows constitutive synthesis of the structural genes thus, the constitutive mutation is cis-acting o operator element.

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