I would like some help understanding how to complete these questions using the f
ID: 64509 • Letter: I
Question
I would like some help understanding how to complete these questions using the following information, since I missed the class that covered this. Thank you.
The growth of the HPV was monitored as follows: 109 cells and 106 viruses in one milliliter were incubated under appropriate conditions. Following a first infectious cycle, a direct count and a plaque assay were performed. In the case of the direct count, the mixture was diluted by a factor of 1000X after which beads were added to obtain a final concentration of 1 X 104 beads/mL. A microscopic field of vision revealed 20 beads and 150 virions. In the case of the plaque assay, 67 plaques were observed on a plate on which 0.1 mL of a 10-5 dilution was assayed.
a) What was the initial multiplicity of infection?
b) According to the direct count, what was the volume observed in a field of vision?
c) According to the direct count, what was the number of virus/mL after the first infection cycle?
d) According to the direct count, what was the burst size after one infectious cycle?
e) What would be the total number of viruses after the last infectious cycle?
Explanation / Answer
A) 109 cells and 106 viruses = Total 1015 was the initial multiplicity of infection.
B)Direct count= 1000mL Volume observed in the field of vision .
C) Direct count = 106 virus/mL
D) Direct count = 1 X 104 beads/mL.
E) total number of viruses after the last infectious cycle will be 150 .