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Please help! Based on Meigs\'s sloppy lab work, you demote him to a position as

ID: 65141 • Letter: P

Question

Please help!

Based on Meigs's sloppy lab work, you demote him to a position as Test tube washer / Errand boy, and resume your DNA sequencing work on your own. You receive a plasmid from a scientific colleague, called pRSET A. See this map showing its partial sequence, printed for its 5'-to-3' strand as normal: You design the following oligonucleotide: 5'- atgtatatctccttcttaaag-3. This oligonucleotide anneals to pRSET A close to (and including) its RBS region. In the empty gel diagram at right, sketch the first 20 DNA bands you would expect to see when using this oligonucleotide to sequence pRSET A.

Explanation / Answer

I would definitely suggest but in the question hardly anything could be visualized. So, it would good enough to upload a Q with a clear view.