Academic Integrity: tutoring, explanations, and feedback — we don’t complete graded work or submit on a student’s behalf.

In this activity you will use RFLP analysis to determine the identity of several

ID: 66019 • Letter: I

Question

In this activity you will use RFLP analysis to determine the identity of several individuals. DNA samples were taken from a family consisting of: Mother, bather. Son, Daughter, Grandparents (GP), and three unrelated individuals (UR). Using what you know about Meiosis and inheritance identify the unmarked lanes from the following options: URI, UR2, UR3, Father, GP1, GP2 Lane 3: Land 4: Lane 5: Lane 7 : Lane 12: Lane 13: Why are the Father and Mother lanes not identical? Why are the sibling lanes not identical? Are the Grandparents maternal or paternal"? Explain.

Explanation / Answer

Lane 7 is father

Lane 12 and 13 - GP1 and 2

Lanes 3, 4,5 are unrelated

-----------------------

The father and mother lanes are not identical because they are from different families with different gene markers.

---------------------

The sibling lanes are not identical because of the process of meiosis. In meiosis, crossing over occurs which gives new combinations to the children.

------------------

The grandparents are paternal as they share the markers with the father and not the mother