Struggling with these questions! Couldn\'t upload lab. If you can help, please e
ID: 66171 • Letter: S
Question
Struggling with these questions! Couldn't upload lab. If you can help, please email me at a_singer95@yahoo.com, and I will send you a copy of the lab. Thank you so much!
1. Which would be the best choice for a genetic transformation experiment: a bacterium, an earthworm, a fish, or a mouse? Give three reasons supporting your choice and explain each one. Use questions 1-4 (p. 2) as a guide to elaborate your answer. (3 pts)
2. In this experiment, what is meant by the “control plate” and what purpose does it serve? (3pts)
3. List and describe two ways in which the plasmid pGLO (specifically the green fluorescence protein, GFP) is being used for scientific or biotechnological applications. (2pts)
4. Once DNA is extracted from an organism, what good is it for? List and describe two applications of DNA extraction technique. (2 pts)
Explanation / Answer
1. Bacteria is the best choice for genetic transformation because of the following reasons:
a) To genetically transform an entire organism, you must insert the new gene(s) to every cell in the organism. So a single celled organism will be a good recepient for genetic transformation, as it contains only one cell which needs to take up the gene.
b) An organism which reproduces quickly. Fast production of the offspring will allow you to quickly assess if the new trait has been passed on.
c) The organism should not produce any toxins or compounds which could make people sick.
d) The organism should grow vigorously in the lab environment.
2.
Experimental Controls
Controls are important in all biological disciplines but they are a necessity in Molecular Biology. The Molecular Biologist can rarely directly observe the processes being studied. We depend on the controls to assure us that our experimental results are valid. Each procedure will have its own type of controls, but each should include an appropriate positive and a negative control.
For each experiment, you should carefully consider the purpose of your experiment and how you are planning to accomplish this purpose.
4.The ability to extract DNA is of primary importance to studying the genetic causes of disease and for the development of diagnostics and drugs. It is also essential for carrying out forensic science, sequencing genomes, detecting bacteria and viruses in the environment and for determining paternity.