You are interested to prepare a 50 ml agarose gel that has a final concentration
ID: 67885 • Letter: Y
Question
You are interested to prepare a 50 ml agarose gel that has a final concentration of 2% (w/v) agarose and 1X SYBR Safe Stain. How much agarose (in grams) and 10,000X SYBR Safe Stain (in microliters) will you use to prepare this gel?
You are interested to prepare a 50 ml agarose gel that has a final concentration of 2% (w/v) agarose and 1X SYBR Safe Stain. How much agarose (in grams) and 10,000X SYBR Safe Stain (in microliters) will you use to prepare this gel?
You are interested to prepare a 50 ml agarose gel that has a final concentration of 2% (w/v) agarose and 1X SYBR Safe Stain. How much agarose (in grams) and 10,000X SYBR Safe Stain (in microliters) will you use to prepare this gel?
Explanation / Answer
1) 2% means 2g in 100ml
We need to prepare 50 ml of agarose gel solution,
So, we need= 2g/2=1g.
So 1 g of agarose is needed to prepare 2% of 50ml agarose gel
2) Preparation of 1X SYBR Safe Stain for 50 ml of agarose gel solution
Dilute 5 µL of 10,000X SYBR Safe Stain concentrate with 50 ml buffer and you will get 1X SYBR safe stain for 50ml agarose gel.