Consider an experiment in which nucleic acids are separated using agarose gel el
ID: 68550 • Letter: C
Question
Consider an experiment in which nucleic acids are separated using agarose gel electrophoresis. In the gel you load the following samples: Lane 1: a population of linearized 3000-bp double-stranded DNA (dsDNA) molecules. Lane 2: a population of circular 3000-bp double-stranded DNA (dsDNA) molecules Which population of nucleic acids would most likely migrate through the gel fastest, and in which direction through the gel will these molecules migrate?
-The linearized dsDNA would most likely migrate faster than the circular dsDNA. All DNA molecules would migrate toward the positive end of the gel.
-The circular dsDNA would most likely migrate faster than the linearized dsDNA. All DNA molecules would migrate toward the positive end of the gel.
-The circular dsDNA would most likely migrate faster than the linearized dsDNA. All DNA molecules would migrate toward the negative end of the gel.
-The linearized dsDNA would most likely migrate faster than the circular dsDNA. All DNA molecules would migrate towards the negative end of the gel.
Explanation / Answer
-The circular dsDNA would most likely migrate faster than the linearized dsDNA. All DNA molecules would migrate toward the positive end of the gel.