Could someone answer both of these questions thoroughly asap please? nput TERRA
ID: 69689 • Letter: C
Question
Could someone answer both of these questions thoroughly asap please? nput TERRA 58% 9% Vigin Figure 2 LWestern blot validation of TERRA-bound RBPs identified by mass spectrometry. (a) Biotinylated TERRA transcripts were incubated with nuclear protein lysates from primary MEFs and their association with the proteins hnRNP A1, A2B1, F, M, D/AUF1 and HuR was detected by western blotting. A biotinylated control RNA (C) corresponding to a random sequence of the same length as the biotinylated TERRA (N48) was used as well. The RBP vigilin was used to monitor the evenness of sample input, as it was identified in both TERRA and control samples. Quantification of the TERRA signal/input ratio was carried out to assess the amount of endogenous RBPs bound to TERRA (see % bound to TERRA). f. Explain why the authors mean by the value assigned to 96 bound to TERRA (2 pts) After close analysis of the RBPs above list the TERRA binding proteins in order of greatest affinity to lowest affinity. (Spts) g.Explanation / Answer
Answer f. The authors mean how much endogenous RBPs bound to TERRA that is what percent of endogenous RBPs is bound to TERRA.They are measuring the amount of RBPs bound in terms of percent.
Answer g. As evident from the image above, the TERRA binding proteins in order of greatest affinity to lowest affinity is as follows: