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II. EXPERIMENTS A. Questions related to: DENOSSTRATOSS 1. Be able to diagram B b

ID: 717005 • Letter: I

Question

II. EXPERIMENTS A. Questions related to: DENOSSTRATOSS 1. Be able to diagram B by drawing the path of a particle of carmine dye in solution. (Daw whtyou sw under microscope) a. Why do the dye particles move this vay? (p.i; A.) b. What is the source of energy to produce this movement? (p.I: A,D) e. How would cooling affect Broming movement? Why? (Thinkt) d. How would heating affect Browning movement? Why? ink) 2. Define osmosis. (p2: A.4 a. what is the primary solute found in syrup? OptA b. Why did the fluid level rise in the tube? Give mechaniam. (Think) c. Which way is water diffusing? KEY? d. What effect would changing temperature have on rate of rise? WEY? (Thik) e. What effect would changing solute concentration have? WHY? (Thik B. Questions related to: RATE OF OSNOSIS a. Can Na+Cl- diffuse across the plasma nenbrane of the RBC? pAtB) Why or why not? Be Specific. b. What is the osmolarity inside 88C7 7:B2 c. What is the osmolarity of .3 M Nac1? (You shouild be know how to do this. If not, sce formula on p.2 d. What is the osmolarity of .15 NaCl? (W e. What is the osmolarity of .075 Nacl? hid £. What is the osmolarity of distilled vater? (hink!-Hint whar solunes are in disilled water) g. Which of the above solutions are isotonic, hypertonic, hypotonic to RBC? (Think!) h. In which of the above tubes would you expect hemolysis? Why? (Thinkt) i. In which of the above tubes would you not expect hemolysis? Why not? (Thiak) j. Define hemolysis. (p48) k. Does osmosis occur during henolysis?After hemolysis? (Think) e255lab4la-cell.255 205 Lab4 page 8

Explanation / Answer

a. It is caused by the movement of the water molecules, they are constantly hitting the colloid particles and jostling them about. Brownian movement has been explained to be due to the unbalanced bombardment of the particles by the molecules of the dispersion medium (water in most cases).

b.The irregular motion of small particles suspended in a liquid or a gas, caused by the bombardment of the particles by molecules of the medium.

c. Brownian motion is linearly dependent on temperarture which means that lowering the temperature will inhibit the motion.

d. Increase in temperature will increase the energy of the molecues thereby imparting more K.E. to other molecules,increasing Brownian motion.