I need to find the concentrations of a \"high\" and \"low\" dose of chlorhexadin

Question

I need to find the concentrations of a "high" and "low" dose of chlorhexadine gluconate.

This is the information I was given: Chlorohexidine gluconate (4% stock solution): "hi" dose is 100 microliters per well, low dose is 10 microliters/well
Note from professor on report: "Please solve the math so you can indicate what % the bacteria were actually exposed to."

I don't know how to do this when working with a 4% stock solution... help... please.

This is what my professor responded when I asked her for clarification:

Yes, I'm asking you to determine the final concentration of the high and low dose that the bacteria were exposed to. That way someone repeating the experiment can use any volume they like without having to do the math (maybe they'd want to wipe a surface etc...). And also then in the results you can say 0.4% of X reduced biofilm by *this* amount.

Explanation / Answer

4% implies 4gm of compound in 100ml of water

So you need to calculate the how much does 100 microliter (High) has, which is equal to 0.004% of Chlorohexidine gluconate and 10microliter (low) would be 0.0004% of Chlorohexidine gluconate

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