Matching. Use each answer only once. One answer per blank. (Some answers will NO

Question

Matching. Use each answer only once. One answer per blank. (Some answers will NOT be used)

An antibiotic which prevents aminoacyl-tRNA binding to the small (30S) ribosomal subunit in prokaryotes to inhibit bacterial protein synthesis. A means of introducing foreign DNA into an organism (such as a plasmid). Many of the non-covalent interactions involving DNA are of this type. A method by which a stretch of DNA between two primers can be amplified via a process of repetitively heating and cooling using a heat resistant enzyme. A method used to make small predictable changes in a DNA sequence for study These "interrupting" DNA sequences are spliced out during transcription in eukaryotes. Using a P-labeled DNA probe complementary to a specific DNA sequence to identify bacterial colonies containing the gene of interest. Cloning and other Molecular Biology techniques are also called: A method developed by Fred Sanger for sequencing DNA. A DNA sequence to which RNA polymerase binds before beginning transcription. The purine rich region of mRNA to which the ribosome binds to begin translation. An antibiotic which binds to the large (50S) ribosomal subunit in prokaryotes to inhibit bacterial protein synthesis. A. dideoxy method B. Introns C. Ionic interactions D. dipole-dipole E. Site-directed Mutagenesis F. Puromycin G. Shine-Dalgarno sequence H. recombinant DNA I. Tetracycline J. Vector K. Polymerase chain reaction (PCR) L. In situ hybridization M. Exons N. Promotor sequence O. Erythromycin

Explanation / Answer

1. Tetracycline

2. Recombinant DNA

3. Ionic intereactions

4. Polymerase chain reaction

5. site directed mutagensis

6. introns

7. Exons

8. in situ hybridization

9. dideoxy method

10.Promoter sequance

11. Shine-Dalgarno sequance

12. Erythromycin

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