Place the following sequence of events for a transamination reaction that requir
ID: 73003 • Letter: P
Question
Place the following sequence of events for a transamination reaction that requires pyridoxal phosphate in the correct order:
1) deprotonation by lysine at the -carbon of the amino acid tethered to pyridoxal phosphate
2) exchange of the amino acid that makes the Schiff base, releasing free lysine
3) hydrolysis via a cabinolamine intermediate, yielding an -ketoacid and pyridoxamine phosphate
4) reprotonation at the -carbon, resulting in tautomerization of the imine carbon atom
5) a second -ketoacid reacting with pyridoxamine to provide a new amino acid
Explanation / Answer
Answer c) Correct sequence of events in transamination reaction; 21435
2) exchange of the amino acid that makes the Schiff base, releasing free lysine
1) deprotonation by lysine at the -carbon of the amino acid tethered to pyridoxal phosphate
4) Reprotonation at the -carbon, resulting in tautomerization of the imine carbon atom
3) hydrolysis via a carbinolamine intermediate, yielding a -ketoacid and pyridoxamine phosphate
5) a second -ketoacid reacting with pyridoxamine to provide a new amino acid
Reason - As we know that transamination reaction is starts via formation of Schiff base which releases a free lysine amino acid. this lysine amino acid leads to deprotonation of amino acid tethered to pyridoxal phosphate. this is followed by re-protonation and hydrolysis steps. at the end, a new amino acid can be formed by -ketoacid.