The following sequence represents the DNA template strand of a gene: 3\' —TAC CG
ID: 7444 • Letter: T
Question
The following sequence represents the DNA template strand of a gene:3' —TAC CGT GTC TCC CTT GAT ATT GAA CTA—5'
nucleotide number 1 5 10 15 20 25
(a) Give the sequence of the mRNA sequence transcribed from this DNA strand and give the amino acid sequence of the protein it encodes.
(b) Give the amino acid sequence of the protein encoded by this sequence after the following mutations have occurred:
• a transition at nucleotide #10
• a transition at nucleotide #12
• a transition at nucleotide #7
• an insertion of a “G” immediately following nucleotide #10
• a three base pair insert (of your choice) starting with base 5
• What kind of mutated (four bases instead of the typical three) “pop” Amino Acid is created by inserting a G between base 14 and 15 of the original RNA strand
Explanation / Answer
a. The DNA strand is:
3' —TAC CGT GTC TCC CTT GAT ATT GAA CTA—5'
The mRNA sequence is:
AUG GCA CAG AGG GAA CUA UAA CUU GAU
The protein sequence is:
Met-Ala-Gln-Arg-Glu-Leu