Consider this amino acid sequence: Amino-Ala-Gyl-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr
ID: 78302 • Letter: C
Question
Consider this amino acid sequence:
Amino-Ala-Gyl-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-carboxyl
1. Give the possible mRNA sequences that you could use to construct it.
2. Give the possible DNA sequences that could be used to produce the above mRNA/
3. If you were synthesizing this protein in and E. Coli cell, which codons might you choose? (Use and E. Coli codon preference chart)
4. If you were synthesizing this protein in a human cell, which codons might you use? (Use a human codon preference chart)
Explanation / Answer
From the chart considering the amino acid sequence for the purpose of finding out the mRNA, the respective DNA and other codon charts.
Amino-Ala-Gl-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-carboxyl
As we know that each amino acids have one or more codons for their protein coding sequence, so here we will tentatively give an example that would suit it.
1. Giving the possible mRNA sequence-
Amino-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-carboxyl
Amino-GCU-GGU-UGC-AAG-AAU-UUU-UUC-UGG-AAA-ACU-UUC-ACC-UCC-UGC-carboxyl
2. Tentative DNA sequence that could have used to produce that mRNA is given below, in here we know that sequence contains ATGC DNA sequence, here Uracil is replaced by Thymine, so G-C, U-A and A-T conversion takes place.
Amino-GCU-GGU-UGC-AAG-AAU-UUU-UUC-UGG-AAA-ACU-UUC-ACC-UCC-UGC-carboxyl
5' CGACCAACGTTCTTAAAAAAGACCTTTTGAAAGTGGAGGACG 3'
3. From the same amino acid sequence using E. Coli codon preference chart I will be using the following sequence
Amino-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-carboxyl
Amino-GCU-GGU-UGU-AAA-AAU-UUU-UUC-UGG-AAG-ACC-UUC-ACA-UCG-UGC-carboxyl
4. From the same amino acid sequence using human codon preference chart I will be using the following sequence
Amino-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-carboxyl
Amino-GCU-GGU-UGU-AAA-AAU-UUU-UUC-UGG-AAG-ACU-UUC-ACU-UCU-UGC-carboxyl