I propose to design a new drug which will act as an inhibitor for an enzyme. If
ID: 791230 • Letter: I
Question
I propose to design a new drug which will act as an inhibitor for an enzyme. If I have used all current information about the mechanism of this enzyme to design this inhibitor and I carefully engineer it with similar chemical properties of the transition state, what type of inhibitor am I attempting to engineer and how will I know if I have succeeded?
a. An uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Vmax b. A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Vmax c. An uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Km d. A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Km
Explanation / Answer
A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Km.Note that: Competitive inhibition is a form of enzyme inhibition where binding of the inhibitor to the active site on the enzyme prevents binding of the substrate and vice versa.
http://faculty.une.edu/com/courses/bionut/distbio/obj-512/Chap9-enzymeinhibition.gif d. A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Km.
Note that: Competitive inhibition is a form of enzyme inhibition where binding of the inhibitor to the active site on the enzyme prevents binding of the substrate and vice versa.
http://faculty.une.edu/com/courses/bionut/distbio/obj-512/Chap9-enzymeinhibition.gif