Analyze the following Assay samples. A. ) Assay 1. (Pictured Above) On this assa
ID: 80213 • Letter: A
Question
Analyze the following Assay samples.
A. ) Assay 1. (Pictured Above)
On this assay, A1 is a positive control, B1 is a negative control, and samples were analyzed in C1, D1 and all of rows 2, 3 and 4.
-Which sample contains the most protein we are looking for?
-Which sample contains the least?
-Please compare the samples in B2 and D2.
-Please compare the samples in A4 and C4.
B. ) Assay 2 (Pictured Above).
Our positive control is in D6, or negative control is in C6 and rows 1 and 2 are empty.
-Which sample contains the most protein?
-Which sample contains the least?
-Compare the samples in A3 and B5.
C. ) Assay 3 (Pictured Above)
This assay is an activity assay for the enzyme amylase (which breaks down starch). We used iodine, which reacts with starch. A1 and B1 are our controls, and our samples are in rows 2, 3 and 4.
-Which well is our positive control for amylase activity?
-Which sample has the most active amylase?
-Compare amylase activity in samples C2 and C3.
0 0 0 0.0000 D C BO 000 A 1 2 3 4 5 6Explanation / Answer
A) On the given assay, A1 is a positive control, B1 is a negative control.
A1 is the positive control with known response and it is compared with the unknown samples for the detection of particular protein present in the samples.
a)Samples in A1and B3 contains the most protein.
b) Sample in B2 contain more protein than sample in D2.
c) Samples in A4 and C4 contain equal amount of target protein.
B) The positive control is in D6 and negative control is in C6.
a)The samples in B4, C5 and D3 contains most amount of protein.
b) The samples in A4, A6 and D4 contains the least amount of proteins.
c) The samples in A3 contain protein as compared to sample in B5.
C) The given assay is an activity assay for the enzyme amylase. Iodine react with starch to form a dark brown /purple color. As amylase break down starch, less and less starch will be present and the color of the solution become lighter and lighter.
a) The well B1 is the positive control for the amylase activity.
b) The samples in D3 and D4 has the most active amylase.
c) The sample in well C2 had less active amylase as compared to sample in well C3.