Stuck on this homework question, any help would be greAt! If answer is helpful I
ID: 811539 • Letter: S
Question
Stuck on this homework question, any help would be greAt! If answer is helpful I will besure to give a good reveiw! Thank you!!
Question You perform column chromatography on a mixture of reaction products which show two spots on TLC. However, under the column conditions you chose those two compounds do not separate. a. One can alter a number of factors in order to improve the resolution of components by column chromatography. For each of the factors listed in the following table. separation can be improved by either by increasing ( ) or decreasing ( ) the factor. For each, describe how changes in sample size or interactions between components and the chromatography medium are altered by ( ) or ( ) the factor so that resolution will be improved. [Answer block continues on next page]. Column Diameter: Column Length: Sample volume: Sample concentration:Explanation / Answer
We are going to say we have compound 1 and compound 2, and we know that inside of the column we have an stationary fase, that is the one that have the active sites that will make that the compoun 1 stay attach to it and the compound 2 passes through been dragged by the movil fase that is the adecuate solvent.
Column diameter:
If you increase the column diameter you will increse the amount of active sites that are in a place so the compund 1 will get stock at the same level. You should be able to make the separation, but you will expend more stationary fase.
If you decrease the column diameter you will need to make the column longger, because you won't have enough site to catch all the compound 1 so you have the mix at the end
Column length:
If you increase the length of the column you will be able to have more active sites that eventually can hold compound 1 and let the other pass, but you will have the compound 1 in a longer part of the column so you have to pass more solvent to dragged all the compound1 wich can make that you a bigger nummber of portions at the end. If you decrease the length of the column you will not have enough active site to catch all the compound 1 so will still have the mix.
Sample volume:
If you increase the sample you will not be able to make the separation, because all the active sites will be taken by an amount of compound 1 and the rest will pass with the compound 2 and you still have the mix at the end. If you decrease the volume you will have a better separation because you will have more active site for lower amount of molecules that need to be catch.
Sample concentration:
Same thing if you increase it you will have the active sites ocuppaid and them won't be enough so compound 1 and 2 will pass together. If you decrease the concentration you will have a better separation, because you have more active site than you need to catch all the compound 1, so if a molecule of compound 1 gets free can be catch down the column for another active site.