In order to measure the substrate dependency effects on initial velocity ( V 0)
ID: 812774 • Letter: I
Question
In order to measure the substrate dependency effects on initial velocity (V0) or rate of an enzymatic reaction; a researcher prepared a
set of reaction flasks (100 mL total volume) and added to each a different concentrations of substrate [S]. He/She added an aliquot a
fixed amount of enzyme and he/she measured the quantity of product formed per unit time (?mol/min.), yielding the following
1. Trace the double reciprocal plot with the data shown above and determine Vmax.
Comment
In order to measure the substrate dependency effects on initial velocity (V0) or rate of an enzymatic reaction; a researcher prepared a set of reaction flasks (100 mL total volume) and added to each a different concentrations of substrate [S]. He/She added an aliquot a fixed amount of enzyme and he/she measured the quantity of product formed per unit time (?mol/min.), yielding the following results: 1. Trace the double reciprocal plot with the data shown above and determine Vmax. CommentExplanation / Answer
From the previous data,
as concentration of substrate [S] increases, the initial velocity increases, probably, the rate of reaction is directly dependent on the concentratino of substrate [S] such as:
rate = k