Part1: You take UV/Vis spectra of two samples. One has an absorbance of 1.2 OD a
ID: 818295 • Letter: P
Question
Part1: You take UV/Vis spectra of two samples. One has an absorbance of 1.2 OD at 500 nm. The other sample has an OD of 0.5 at 500 nm. Both have an overall orange color. Which solution is darker?
Both samples are about the same depth of color; this is not a significant difference
Cannot be determined from the data provided
0.30 OD sample is darker
1.20 OD sample is darker
spin
transition
electronic
chemical
Vibrational
A.Both samples are about the same depth of color; this is not a significant difference
B.Cannot be determined from the data provided
C.0.30 OD sample is darker
D.
1.20 OD sample is darker
Explanation / Answer
1) D
1.2 OD sample is darker.
Since OD = Absorbance = e l x C where e is const of sample and hence same for both.
, l = cell path length ( same for both)
hence sample having 1.2 OD will have more concenration than solution with OD 0.5
More is concnetration darker is sample.
2) C
- Elctronic tranisons.
UV/VIs spectroscopy deals with elctron transitions from sigm or pi ponds to excited sigma or pi bonds.