You pick one of your auxotrophic mutants and test it for what nutrient it needs

Question

You pick one of your auxotrophic mutants and test it for what nutrient it needs to grow. So that you don't have to do 20 different plates, you use a pooled nutrient strategy. Yeast are put on minimal media supplemented with the following nutrient pools. (Each row and column is a separate pool. So e.g., pool 1 contains alanine, tryptophan and phenylalanine, while pool 4 contains alanine, guanine and threonine.) This mutant colony grows on media supplemented with either nutrient pool 2 or 5. What single nutrient does this demonstrate that the mutant is auxotropic for? Explain your reasoning.

Explanation / Answer

Auxotroph is a mutant organism (typically a bacterium or fungus) that requires a particular additional nutrient that the normal strain does not.

So for mutant colony grows on media supplemented with nutrient pool 2 and 5 signify organism is auxotroph for valine.

Reason: Auxotrophic strains will only grow on media supplemented with the required molecule. As valine is present in both, therefore, organism is auxotroph for valine.

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