Analyze the Forensic DNA data. Answer the following questions and determine the
ID: 82724 • Letter: A
Question
Analyze the Forensic DNA data. Answer the following questions and determine the identity of the persons inside the shark. Use what you know about patterns of inheritance to make your decisions.
Sample Codes:
01=Tissue remains found in shark, 04=Daughter of missing fisherman#1
02= Bone remains found in shark, 05= Son of missing Fisherman #1
03=Tissue remains found in shark, 06= Mother of missing Fisherman# 2
07= Half Brother of missing Fisherman #2
1) There are 2 missing fishermen.Multiple human remains were found in the shark. Are the remains (samples 01, 02. and 03) from one person,two or three? show your evidence.
2) a. Samples 04 and 05 are from the children of missing Fisherman #1. If samples 01 or 02 are from fisherman #1, what STR variations would you expect to see his children inherit?
b.Do any of the 16 STR loci suggest that samples 01 and 02 are from the father of the children in columns 04 and 05?
c. which of the 16 STR loci suggest that sample 01 and 02 are not from the father of the children in columns 04 and 05?
Summary of STR Results. Sample 01 06 07 (Tissue) (Bone) (Tissue) (Reference) (Reference) (Reference) (Reference) Locus 14.16 15 16 16 14, 16 15, 17 D3S1358 TH01 6,93 6,93 7, 9.3 29.30 30, 31.2 29, 30 D21S11 29, 30 30, 31 29, 32.2 12,16 12,16 17,19 12, 13 12, 16 D18S51 16, 18 INC 89 17h 13 9, 11. 13.15 Penta E D5s818 8, 10 8, 10 11,12 10, 11 13 12, 13 11,12 13 1213 11 11, 12 D13S317 11, 12 11 9,12 9, 10 10, 12 D7S820 10, 11 10, 11 NR INC 8,9 s, 11 11, 12 D16S539 10, 11 10, 12 CSF1PO 11, 12 INC 2.2,9 Penta D Amelogenin VWA 15, 15, 19 14, 16 16, 19 14, 16 15 15 13,15 14, 15 14, 15 12 D8S1179 (8,9 8,9 TPOX FGA 24,25 22,25 2,24 21,25 24, 25 20, 24 *peak(s) present below reporting threshold; locus not used for comparison peak imbalance present INC locus inconclusive, not used in comparison INR no results Sample 07 represents the fisherman's half-brother; he shares the same father but has a different mother.Explanation / Answer
Samples 01 and 02 are identical except for the part where athere are missing data points. This suggests that the remains are from one person.
The children should each have one of the father's variation for evert STR locus.
The variations atr TH01, D21S11, D8S1179, and TPOX are consistent with Fisherman 1 being the father.
The variations at the other STR alleles do not match as expected, i.e. there are variations in the children that are not present in the father. Eg. neither child has variation 8 or 10 at D5S818 that are presenr in Fisherman 1.