In DNA replication on the leading strand, what is the order enzymes are needed t

Question

In DNA replication on the leading strand, what is the order enzymes are needed to complete the process correctly? Topoisomerase > Helicase > Primase DNA polymerase lll > DNA polymerase l > DNA ligase, repeated for each Okazaki fragment Topoisomerase > Helicase > Primase > DNA polymerase lll > DNA polymerase l > DNA ligase None of the above Topoisomerase > Helicase > Primase DNA polymerase l > DNA polymerase lll > DNA ligase Helicase > Topoisomerase Prim > DNA polymerase l > DNA polymerase lll > DNA ligase

Explanation / Answer

Helicase>topoisomerase>primase>DNA polymerase1>DNA polymerase 3>DNA ligase

Helicase functions in opening the double stranded DNA after which topoisomerase relieves the torsional stress caused by supercoiling on the other side.Primase enzyme adds the primers so that construction of lagging strand can begin by using leading strand as template. DNA polymerase1 removes the primer and extends the strand in the 5->3 direction. It is also involved in proofreading the strands. then DNA polymerase 3 comes into actions but it can add nucleotides only to the 3 end . Finally ligase functions to join the two strands to form ds DNA

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