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2 https://session.masteringchemistry.com/myct/itemView?assignmentProblemID-49056987 CHEM 3510 Summer 2015 Signed in as Yajaira Peralta Help Close Problem 4.6 Resources « previous 15 of 15 return to assignment Using the pKa in the table below and the Henderson-Hasselbalch equation, calculate the approximate net charge on each of the four common ribonucleoside 5-monophosphates (rNMPs) at pH 3.8. If a mixture of these rNMPs was placed in an electrophoresis apparatus, as shown, draw four bands to predict the direction and relative migration rate of each TABLE Ionization con stants of ribonucleotides expressed as pKa Values Phosphate Base Primary lonization Secondary lonization HO-P-RHO-PR OH Reaction (as Loss of Proton from) pKa2 6.1 6.1 pKa 3.8 2.4 9.4 9.5 4.5 5' AMP 0.9 N-1 5' GMP 0.7 N-7 5' UMP 1.0 6.4 N-3 5' CMP 0.8 6.3 N-3 Drag the appropriate labels to their respective targets CMP AMP UMP GMP Application zone

Explanation / Answer

Hello! from Left to right it would be: GMP, AMP, CMP and UMP. Regards!

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