Please explain 1. a) lved into Methylene Chloride a Silica Chromatography Column
ID: 887318 • Letter: P
Question
Please explain
1. a) lved into Methylene Chloride a Silica Chromatography Column. After 6L of n-Hexane is added to the column the yellow band of Fluorenone has only moved down the column slightly. What can be done to improve this experiment? A Protion of the Saca Matrix n-Hexane Methylene Chioride Fluorenone b) luorenone is more polar than Fluorene, which will elute off of your very polar Alumina column first when using hexane as the eluting solvent? Why? (One point given for the why" portion of the answer) FluoreneExplanation / Answer
a) for improve the experiment you can have a true "control" group (no variables), do multiple experiments for each group (3-5), so you can use a much more accurate average result; use properly calibrated equipment;
have multiple test groups (0% concentration "control", 25%, 50%, 75%, 100%, etc)
b) Fluorenone is more polar than fluorene because of its C=O bond. Fluorenone's spot will be lower than fluorene because of its "stickness." Fluorenone will be absorved to the alumina more strongly. Fluorene will elute off the column with a nonpolar solven hexane, whereas fluorenone will not come off until a more polar solvent is put on the column. So, the Fluorene will elute off first.