Academic Integrity: tutoring, explanations, and feedback — we don’t complete graded work or submit on a student’s behalf.

The data below show the binding of oxygen to squid hemocyanin. [O2] fB x100 [O2]

ID: 909209 • Letter: T

Question

The data below show the binding of oxygen to squid hemocyanin.

[O2]

fB x100

[O2]

fB x100

1.13

0.30

136.7

55.7

5.55

1.33

166.8

67.3

7.72

1.92

203.2

73.4

10.72

3.01

262.2

79.4

31.71

8.37

327.0

83.4

71.87

18.96

452.8

87.5

100.5

32.90

566.9

89.2

123.3

47.80

736.7

91.3

Determine from a Hill plot whether the binding of oxygen to squid hemocyanin is non- cooperative, fully cooperative or partially cooperative. Determine the MINIMUM number of binding sites consistent with the data.

[O2]

fB x100

[O2]

fB x100

1.13

0.30

136.7

55.7

5.55

1.33

166.8

67.3

7.72

1.92

203.2

73.4

10.72

3.01

262.2

79.4

31.71

8.37

327.0

83.4

71.87

18.96

452.8

87.5

100.5

32.90

566.9

89.2

123.3

47.80

736.7

91.3

Explanation / Answer

The data below show the binding of oxygen to squid hemocyanin. [O2] fB x100 [O2]