Match the spectrophotometer part with its description. Match the spectrophotomet
ID: 909496 • Letter: M
Question
Match the spectrophotometer part with its description.
Match the spectrophotometer part with its description. Reference Cuvet Detector Light Source Scanning monochromator Mirror Amplifier Beam Chopper Sample Cuvet Houses the reagent blank. Passes the beam to the detector. Holds the sample with a defined pathlength. Increases the detector signal for ease of display and quantitation. Mirror that rotates to direct light to either the sample of the reference cuvette. Provides electromagnetic radiation in the UV, Vis, or IR region of the spectrum. Typically a photomultiplier tube that generates an electrical current proportional to the photon flux. Wavelength selector that passes a narrow band of wavelengths to the sample by scanning through a user-defined range of selected wavelenthsExplanation / Answer
Answers:
1. Reference cuvet: houses the reagent blank.
It contains the reference solution/blank solution which is devoid of the analyte and has only the solvent.
2. Mirror: passes the beam to the detector.
Various mirrors are employed to pass the light to the detector.
3. Sample cuvet: holds the sample with a defined pathlength.
It coantains the analyte to be analysed.
4. Amplifier: increases the detector signal for ease of display and quantitation.
It amplifies the weak intensities of radiation passing through the cuvet.
5. Beam chopper: mirror that rotates to direct the light to either the sample or the reference cuvet.
It is a rotating mirror employed to divide the incident light into two so that it passes both cuvets (reference ans sample).
6. Light source: provides electromagnetic radiation in the UV,Vis or IR region of the spectrum.
It provides the desired radiation (e.g tungsten lamp, halogen discharge lamp)
7. Detector: typically a photomultiplier tube that generates an electrical current proportional to the photon flux.
It detects the transmitted radiation and calculates the ansorbance.
8. Scanning monochromator: wavelength selector that passes a narrow band of wavelengths to the sample by scanning through a user defined range of selected wavelengths.
Monochromator separates the radiation into into its constituent wavelengths and provides the desired wavelength for analysis.