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In yeast, ethanol is produced from glucose under anaerobic conditions. What is t

ID: 916435 • Letter: I

Question

In yeast, ethanol is produced from glucose under anaerobic conditions. What is the maximum amount of ethanol (in millimoles) that could theoretically be produced under the following conditions?

a. A cell-free yeast extract is placed in a solution that contains 2.50 × 10^2 mmol glucose, 0.40 mmol ADP, 0.40 mmol Pi, 0.80 mmol ATP, 0.20 mmol NAD , and 0.20 mmol NADH. It is kept under anaerobic conditions. Under the same conditions, what is the theoretical minimum amount of glucose (in millimoles) required in the solution to form the maximum amount of ethanol?

b. Under the same conditions, what is the theoretical maximum amount of glucose (mmol) required in the solution to form the maximum amount of ethanol?

Explanation / Answer

C6H12O6 -------------> 2C2H5OH + 2 CO2

hence one molecule glucose will 2 molecule of Ethanol

glucose concentration = 2.50 × 102 mmol

                                 = 2.50 × 102   x 180.16 = 4.5 x 104 gm or 45 kg

Maximum it can produce (2.50 × 102 x 2) 5 x 102 mmols of ethanol

                                5 x 102 mmols of ethanol   =   5 x 102 x 46.068 = 2.3 x 104 gm or 23 kg of ethanol

Hence Minimum 45KG glucose is need to produce 23KG ethanol