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Question 9 of 16 Map sapling learning A student researcher performed a chromatog

ID: 916825 • Letter: Q

Question

Question 9 of 16 Map sapling learning A student researcher performed a chromatographic separation of caffeine and aspartame with methanol as the mobile phase using a C-18 column. The retention times for methanol (tm), caffeine (c), and aspartame (t) were 40.0 s, 100.7 s, and 177.2 s, respectively. A) What is the selectivity factor (relative retention), a, for this column? Number B) Calculate the hypothetical retention times for caffeine and aspartame if their retention factors were reduced by 34.0%. Assume the mobile phase retention time (tm a 40.0 s) remains constant. Number Number eine aspal C) Choose the statements that correctly describe the outcome if the partition coefficient for caffeine (Kc) were to decrease (Hint: there is more than one correct answer). The retention factor of caffeine would decrease n The retention time of caffeine would increase. The retention time of caffeine would decrease. The retention factor of caffeine would increase Caffeine would spend a greater amount of time in the mobile phase. The retention time and retention factor of caffeine would not change.

Explanation / Answer

Question 9 of 16 Map sapling learning A student researcher performed a chromatog