Can someone help me with this problem? I\'m not sure if its right. The protocol
ID: 917647 • Letter: C
Question
Can someone help me with this problem? I'm not sure if its right.
The protocol for this exercise stresses the need to use similarly shaped proteins as standards when using gel permeation chromatography to estimate the molecular weight of a protein. Why is shape important? If the protein is, for example, a disk shaped molecule, sort of like an M&M; candy, the molecules will settle on top of the gel beads and have a hard time getting through, requiring a much larger volume of buffer to elute than would be predicted from the molecular weight If a protein is rod-shaped, it can turn on end and slide down the column very fast (avoiding the beads), eluting at a much lower volume than its molecular weight would suggest. The elution volume of a protein is actually a reflection of the apparent volume that the molecule occupies, and its resulting effective cross-sectional area, not its molecular weight. This is not a concern for proteins. It is only a concern for other molecules, such as DNA, which is a long, more or less rod shaped molecule. All proteins are roughly the same shape.Explanation / Answer
Yes its correct answer.
Actually there are some important factor to consider while determining the mw of protien with the help of gel chromatography. gel selection, selection of standard protein that of similarly shsape.