Consider the following uv/vis absorption spectrum for a solution of the nucleoca
ID: 920541 • Letter: C
Question
Consider the following uv/vis absorption spectrum for a solution of the nucleocapsid protein NCp15. What is the preferred wavelength at which to measure calibration standards and determine the concentration of a dilute solution of the protein? 313 nm, because the high molar absorptivity at this wavelength will yield a calibration with the greatest sensitivity 352 nm, because the large slope in the absorption spectrum at this wavelength will result in a calibration plot with the greatest sensitivity 700 nm, because the low, stable value of absorbance at this wavelength will yield the most linear calibration plot Quantitation should not be performed at a single wavelength, but should instead be performed by scanning the entire uv/vis region.Explanation / Answer
5(a) 313 nm, because the high molar absorptivity at this wave length will yield with the greatest sensitivity