Hey everyone, I am currently undertaking independent research in which I grow a

Question

Hey everyone,

I am currently undertaking independent research in which I grow a strain of bacteria in different types of agre to calculate cell growth using fractional analysis. So this includes a box count method obviously.

This lab really includes both knowledge of Microbio and Physics. But I had a problem, and I was hoping somebody here with some vast knowledge could lead me in the right step of taking a CLEAR picture.

I am using a program called "FracLac" for use of box counting http://rsbweb.nih.gov/ij/plugins/fraclac/FLHelp/UseFracLac.htm

I need to get a clearer picture of our bacteria so that we can achieve more accurate of a box count for our calculations. We have tried using a microscope and taking pictures, and even using a scanner (as done in similar research in the 90's by Japanese scientists). However, the quality is not what I am looking for.

Does anybody know a method in which I can take a very good quality picture of the bacteria? I would appreciate anything that is feasible. If you offer me an idea that would be impossible to achieve though, like getting an electron microscope or something, please let me know. I accept all ideas.

Thank you so much.
http://biocurious.com/images/fractalBacteria.jpg
^ that is the kind of HIGH quality I want. I REALLY want defined pictures, as little fuzzy as possible.

Explanation / Answer

To get a clear picture of the bacteria, advanced techniques has to be used. The Fraclac can be used to count the number of bacteria, but it cannot provide a clear picture of image. To obtain a clear picture of bacteria th efollowing techniques can be used, depending on the type of purpose. -Advanced transmission electron microscopy. - Electron tomography. -High resolution transmission eletctron transmission. - Morphometry and -Flurometry Computer processed microscopic images can be good quality and clear. -Marr - Hildreth and edge strength technique can also be used. Hope it helps.

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