Could I please get an A, B, C, D, E response for these questions (detail isnt ne
ID: 93381 • Letter: C
Question
Could I please get an A, B, C, D, E response for these questions (detail isnt necessary)? Thank you.
Protein X, had a mass of 60 kilodalton (kDa) from gel-filtration chromatography. In the presence of urea, the same protein yielded a molecular species Y, with a mass of 30 kDa. Repeating the chromatography in the presence of urea and -mercaptoethanol, resulted in a single molecular species Z, of mass 15 kDa
1) What happens to the protein when treated with urea?
A. There is no effect.
B. Ion pair (salt bridge) interactions are disrupted.
C. Disulfide bonds are disrupted.
D. Noncovalent interactions are disrupted.
E. Peptide bonds are disrupted
2) How many copies of protein X are present in protein Y?
A. 1
B. 2
C. 3
D. 4
E. None of these.
3) How many subunits are present in protein Z?
A. 0
B. 1
C. 2
D. 3
E. 4.
4) How is protein Y assembled from Protein X?
A. By ion pair (salt bridge) interactions.
B. Noncovalent interactions.
C. Hydrophobic interactions.
D. Disulfide bond formation.
E. none of the above.
Explanation / Answer
Ans 1: when protein is treated with urea it's nin covalent interactions are disrupted. So option D is correct
Ans 2: since protein X yields Y. So option E is correct.