Format Tools Table Window Help Week 11 Post ailings Review view References A. A
ID: 93593 • Letter: F
Question
Format Tools Table Window Help Week 11 Post ailings Review view References A. A AaBbCcDd Normal Week 10 Restriction en Post-Lab Questions Name: 1. In today's lab, you purified plasmid DNA from E. coli. What types of molecules are present in the E. coli that you started with? 2. After the purification what types of molecules do you expect to have in your sample? 3. What restriction enzyme did you use for the digest today? 4. what size(s) of DNA fragments do you expect to get from the restriction enzyme digest if your bacteria contain the plasmid with no insert? 5. What size(s) of DNA do you expect to get from the restriction enzyme digest if your bacteria contain the plasmid with the gene of interest? 6. Based on what you know about how restriction enzymes work explain why you are able to predict the sizes of DNA that will be obtained from this experiment. sh (USExplanation / Answer
1: proteins, RNA, and DNA are the major biomolecules that are being separated during DNA isolation.
2: DNA is expected to be present in sample.
3: specific name of restriction enzyme is not mentioned in this protocol so more information is needed.
4: more information is required.
5: more information is required.
6: because DNA has particular size and restriction enzyme do cut at particular site. So by knowing the DNA size and the sequence of restriction site being cut by restriction enzyme we could determine the size of both DNA fragment as well as the insert.