The following sequence of DNA is transcribed and encodes a small protein with th
ID: 94440 • Letter: T
Question
The following sequence of DNA is transcribed and encodes a small protein with the sequence Met Tyr Arg Gly Ala. 5'-CCTATGCCCGCATCATTACGCACCTCTGTACATGGC-3' 3'-GGATACGGGCGTAGTAATGCGTGGAGACATGTACCC-5' a) Which strand of DNA is the template strand for transcription? Which direction (right to left or left to right) does RNA polymerase move along the template as it transcribes this gene? b) What is the sequence of the nucleotides in the processed mRNA molecule for this gene? Indicate the 5' and3' directions of this gene. c) A single base mutation in the gene results in synthesis of the peptide Met Tyr. What is the sequence of nucleotides making up the mRNA produced by this mutant gene? List three characteristics of the genetic code. How can you distinguish experimentally among DNA polymerase III, RNA polymerase, and primase? Suppose you isolate a mutant bacterium that does not have an active ligase enzyme. Can this bacterium replicate? Why or why not? At what step will replication be halted?Explanation / Answer
a upper strand is the template strand.
It moves left to right
b. Reolace C with G A with U T with A and G with C
2 Specificity , Universal and Redunanat