The figure depicts the primary structure of an enzyme. The amino acids that make
ID: 95736 • Letter: T
Question
The figure depicts the primary structure of an enzyme. The amino acids that make up the active site are marked for the wild type enzyme (top amino acids) and a mutant enzyme (bottom amino acids). Based on this, what is your BEST prediction about the mutant enzyme? T F ? Mutant enzyme will have lower activity: will not bind substrate as well as Leu, and will not stabilize the transition state. T F ? Mutant enzyme will have lower activity: mutation will cause protein to unfold. T F ? Mutant enzyme will have the same activity as wild type: Leu and have similar R-groups with similar weak bonds.Explanation / Answer
1) True. The change in the enzyme from leucine to isoleucine will alter the activity of the enzyme because one of the amino acids has changed then the binding of substrate will be changed.
2) false- it will not cause the enzyme to unfold because of the polarity is same on the R group.
3)false- it will alter the activity of enzyme.