Serine proteases stabilize the transition state in order to effectively increase
ID: 966175 • Letter: S
Question
Serine proteases stabilize the transition state in order to effectively increase the rate of the reaction. Which panel represents the transition state?
Panel A (Enzyme-substrate complex)
Panel B (Tetrahedral intermediate)
Panel C (Acyl-enzyme intermediate)
Panel D
Panel F (Active enzyme)
A.Panel A (Enzyme-substrate complex)
B.Panel B (Tetrahedral intermediate)
C.Panel C (Acyl-enzyme intermediate)
D.Panel D
E.Panel F (Active enzyme)
Asp 102 His 57 Asp 102 57 CH2 CH2 Ser 195 Ser 195 N 1 CH2 CH2 Nucleophilic attack R' Substrate/ polypeptide N Tetrahedral intermediate Enzyme-substrate complex Asp 102 57 Asp 102 His 57 CH2 Ser 195 CH2 Ser 195 H20 N 1 CH2 CH2 New N-terminus of R cleaved polypeptide R chain R'NH2 N C 0 Acyl-enzyme intermediate Asp 102 His 57 Asp 102 His 57 CH2 Ser 195 CH2 Ser 195 CH2 CH2 New C-terminus of cleaved polypeptide/R chain Tetrahedral intermediate Active enzyme © 2008 John Wiley & Sons, Inc. All rights reserved.Explanation / Answer
b ) Panel B ( Tetrahedral intermediate)
Serine proteases preferentially bind the transition state and the overall structure is tetrahedral and this couse lowering the activation energy of the reaction.