I need in detail mechanism so I\'ll be able to do the same on my examination. Pl
ID: 972093 • Letter: I
Question
I need in detail mechanism so I'll be able to do the same on my examination. Please and thank you 1. Give an example of general acid catalysis. Use a chemical reaction to illustrate your point. 3. Give an example of nucleophilic catalysis. Use a chemical reaction to illustrate your point. 4. Provide an example of metal ion catalysis using a chemical reaction. Use a chemical reaction to illustrate your point. 5. What enzymes are examples of serine proteases and why? 6. Explain the substrate selectivity among these enzymes. 7. What amino acids play an important role in the catalytic mechanism of chymotrypsin? 8. Consider the substrate Ala-Pro Gly- Phe-Gly. Provide the full catalytic mechanism for the cleavage of this substrate by chymotrypsin. Be sure to show all intermediates and use proper curved arrow notation. 9. What is meant by burst kinetics? Provide an example 10. What is p-nitrophenol acetate and why is it a good substrate for studying chymotrypsin 11. What is TPCK? Draw its structure and illustrate how it reacts with chymotrypsin. Use proper curved arrow notation. 12. When during the course of a reaction are the most contacts made between an en and the substrate, zyme 13. What is a transition state analog? Why are these compounds potent enzyme inhibitors?Explanation / Answer
9) burst kinetics is a enzyme kinetics which refers the intial high velocity of an enzyme turnover when adding enzyme to substrate.the period at which where the high velocity product is formed is called as burst kinetics or burst phase.
ex: in glycolysis processb at sixth step ivolving GAP Dehydrogenase and the reduction of NAD to NADH
11) TPCK MEANS TOSYL PHENYLALANYL CHLOROMETHYLKETONE