In eukaryotic DNA replication, DNA polymerase has a primase activity and relativ
ID: 974233 • Letter: I
Question
In eukaryotic DNA replication, DNA polymerase has a primase activity and relatively low processivity, and DNA polymerase has a 3’ to 5’ proofreading activity, lacks a primase activity, and has a very high processivity when complexed with PCNA. Both enzymes replicate DNA by extending a primer in the 5’ to 3’ direction under the direction of a single-stranded DNA template.
a). Explain why DNA polymerase may act as the leading-strand polymerase and the DNA polymerase could be the lagging strand synthesizing enzyme.
Explanation / Answer
At least three DNA polymerases are required for eukaryotic genome replication: DNA polymerase alpha (Pol ), DNA polymerase delta (Pol ) and DNA polymerase epsilon (Pol )DNA polymerase could be the lagging strand synthesizing enzyme. Because its primary function in DNA replication is to create many short DNA regions rather than a few very long regions. DNA polymerase may act as the leading-strand polymerase. It is the polymerase enzyme primarily responsible for DNA replication. It assembles into a replication complex at the replication fork that exhibits extremely high processivity, remaining intact for the entire replication cycle.