Indicate how each of the following will affect an enzyme-catalyzed reaction if t
ID: 974522 • Letter: I
Question
Indicate how each of the following will affect an enzyme-catalyzed reaction if the enzyme has an optimum temperature of 37 DegreeC and an optimum pH of 8: decreasing the temperature of the reaction mixture The rate of catalysis will slow or stop. The rate of catalysis will increase significantly. The rate of catalysis will remain the same. adjusting the pH of the reaction mixture to pH 5 The rate of catalysis will slow or stop. The rate of catalysis will increase significantly. The rate of catalysis will remain the same. adjusting the pH of the reaction mixture to pH 10 The rate of catalysis will slow or stop. The rate of catalysis will increase significantly. The rate of catalysis will remain the same.Explanation / Answer
Enzymes have optimum temperature and pH needed for its activity. Above and below of these optimum values the activity of every enzymes will reduce significantly.
For an enzyme with optimum temperature 37oC , the reduction in temperature to 15oC will reduce the activity. Because activation energy will be less at reduced temperature, making the enzyme unable to catalyse. So the answer is rate of catalysis will slow or stop.
Adjusting pH to 5 will also cause the rate of catalysis to slow or stop. Enzymes are proteins made of aminoacids which require an optimum pH to stabilise the structure. So changing the pH from their optimum values even denature the proteins.
Adjusting the pH of the reaction mixture to 10 will cause the rate of catalysis to slow or stop. Reason as given above.